A DH population comprising 120 DH lines, which was established in
our laboratory, was used in this study. This population was developed
by anther culture of an F1 hybrid between the typical japonica CJ06
and the typical indica TN1 (Sogawa et al., 2004). And 35 conventional
rice varieties were used to evaluate the soluble sugar content in grain.
All materials were planted at the experimental farm of China National
Rice Research Institute (Hangzhou, China) in 2012. Each line was
planted in four rows, with six plants in each row. After harvesting, the
grains of each line with three replicates were sampled for evaluation
of soluble sugar, sucrose and fructose.