Total brain extracts (50 μg/lane) were separated on a 12% sodium dodecyl sulfate (SDS)/polyacrylamide gel and transferred onto PVDF membranes (Millipore, Billerica, USA).
Extract the whole brain (50 μg / lane) were separated. 12% sodium dodecyl (SDS) gel polyacrylamide. And transferred to the material PVDF (very Billerica, USA).
All brain extracts (50 μ g / lane) was split into 12% sodium dodecyl sulfate (SDS polyacrylamide gel ) / plate and transferred to PVDF (Millipore, United States, United States).