For this purpose, the MPNDPCR
estimation of the number of cells was compared with
the direct count. For quantitative analysis, 107 cells in 50 ll
(2 · 108 cells ml)1), as determined by direct cell counts,
were added to the initial tubes of five replicate dilution
series. The cell concentration in the original suspension as
estimated by MPN-DPCR was 1Æ58 · 108 cells ml)1 (95%
confidence limits ¼ 4Æ80 · 107 to 5Æ23 · 108).