The evaluation of bacterial biofilm formation on the surface of PLA and PHB composite films
Fragments 0.5 1.5 cm of the control and test films (with and without PHMG derivatives) were sterilized with ethyl alcohol and placed in sterile Petri dishes with 10 cm3 nutrient broth (composition [g/dm3]: peptone e 5.0, meat extract e 3.0, pH 7.4). 10 ml suspensions of the appropriate bacterial strain with optical density of 1.0 according to Mc Farland standards were then transferred to Petri dishes and incubated at 35 C for 48 h. Subsequently, bacterial biofilm formation on the surface of all film samples was assessed using spectrophotometric measurement of the adsorbed crystal violet.