A loopful of P. taetrolens from a fresh Nutrient Broth agar plate
was used to inoculate a 500 mL Erlenmeyer flask containing
100 mL of Nutrient Broth medium. This flask was incubated on
an orbital shaker (New Brunswick Sci., NJ, USA) at 250 rpm and
30 C for 10 h. Actively growing cells from this culture were subsequently
employed as inoculum for bioreactor seed cultures or
shake-flask cultivations on whey.