.3. Chemical analyses
Standard methods of analysis (AOAC, 2000) were used to determine
crude protein (Official Method No. 950.36), fat (Official Method
No. 935.38), cellulose (Official Method No. 950.37), ash (Official
Method No. 930.22) and moisture contents (Official Method
No. 926.5). In the protein determination, a nitrogen-to-protein
conversion factor of 6.25 was used. Total carbohydrate content
was calculated as a sum of reducing sugar and starch contents
determined by the AOAC Official Method No. 975.14 and ICC Standard
No. 123/1 (1994), respectively.