The DPPH• solution was adjusted with methanol to an absorbance of 0.700 (±0.020) at 515 nm in a 3 mL capacity cuvette (1 cm length) at 25 °C (Lambda 25 UV–VIS spectrophotometer,
The DPPH• solution wasadjusted with methanol to an absorbance of 0.700 (±0.020) at515 nm in a 3 mL capacity cuvette (1 cm length) at 25 °C (Lambda 25UV–VIS spectrophotometer,