The
extruded products were ground previously and then prepared to
10 g solid 100 g1 dispersion using deionized water. Aliquots (25 g)
of homogenized samples were adjusted to pH 2.0 with 4 mol L1
HCl and after addition of 0.8 mL pepsin digestion mixture
(16 g 100 mL1 pepsin solution in 0.1 mol L1 HCl), were incubated
at 37 C for 2 h in a shaking water bath. At the end of pepsin
digestion, dialysis bags (cut off: 8e6 kDa) containing 20 mL
0.15 mol L1 PIPES buffer were placed in each flask and were
incubated for 50 min in a shaking water bath at 37 C. Then, 6.25 mL
of pancreatin solution (0.4 g 100 mL1 pancreatin in 0.1 mol L1
NaHCO3) was added to each flask and the incubation continued for
another 2 h. Then, bag contents dialyzates from M and MPc2
extruded products were weighed and named MD and MPc2D,
respectively.