Phylogenetic analysis of strains from the 13 medicinal
plants was investigated. Their sequences were deposited in
GenBank, their accession numbers were listed in Table 1. The
partial sequence of 16S rRNA gene of Escherichia coli was
used as outgroup in neighbor-joining phylogenetic analysis.
The sequences were first submitted to National Center for
Biotechnology Information (NCBI) to obtain the closest
phylogenetic relatives in GenBank database (http://www.ncbi.
nlm.nih.gov/) using BLASTn version 2.2.21+ [24]. All the
sequences were then aligned in Clustal W [25] and edited into a
multiple alignment file in BioEdit version 1.0 [26]. The
neighbor-joining phylogenetic tree was finally constructed
with MEGA version 4.0 bootstrap analysis [27].