Genetic mapping
Genetic mapping is used to determine the relative location of a particular mutation in the
genome. Genetic mapping is based on the counting of recombination events between the
mutation of interest and genetic markers with known chromosomal locations. Normally, a TDNA
insertion should be genetically linked to one location in the genome, i.e. the location
of the disrupted gene as indicated by DNA sequencing of the T-DNA/plant DNA junctions
at the T-DNA insertion point (Sessions et al., 2002). If there is a translocation associated
with a T-DNA insertion, the T-DNA element will be genetically linked to mapping markers
on two chromosomes (Burnham, 1930; Castle et al., 1993). Linkage will be observed
between the T-DNA and markers near the expected T-DNA insertion site as well as markers
located on a second chromosome near the translocation break-point.
Genetic mappingGenetic mapping is used to determine the relative location of a particular mutation in thegenome. Genetic mapping is based on the counting of recombination events between themutation of interest and genetic markers with known chromosomal locations. Normally, a TDNAinsertion should be genetically linked to one location in the genome, i.e. the locationof the disrupted gene as indicated by DNA sequencing of the T-DNA/plant DNA junctionsat the T-DNA insertion point (Sessions et al., 2002). If there is a translocation associatedwith a T-DNA insertion, the T-DNA element will be genetically linked to mapping markerson two chromosomes (Burnham, 1930; Castle et al., 1993). Linkage will be observedbetween the T-DNA and markers near the expected T-DNA insertion site as well as markerslocated on a second chromosome near the translocation break-point.
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Genetic mapping
Genetic mapping is used to determine the relative location of a particular mutation in the
genome. Genetic mapping is based on the counting of recombination events between the
mutation of interest and genetic markers with known chromosomal locations. Normally, a TDNA
insertion should be genetically linked to one location in the genome, i.e. the location
of the disrupted gene as indicated by DNA sequencing of the T-DNA/plant DNA junctions
at the T-DNA insertion point (Sessions et al., 2002). If there is a translocation associated
with a T-DNA insertion, the T-DNA element will be genetically linked to mapping markers
on two chromosomes (Burnham, 1930; Castle et al., 1993). Linkage will be observed
between the T-DNA and markers near the expected T-DNA insertion site as well as markers
located on a second chromosome near the translocation break-point.
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