Explant Source and Preparation:
Healthy and juvenile shoots of 1-15 cm were collected from donor Paederia foetida plants (2 years old),
grown in the Garden of Medicinal Plants at the Department of Biotechnology and Genetic Engineering,
University of Development Alternative and were used in the study. Leaves were trimmed and single nodes
were excised with a sharp knife. The single node explants were surface sterilized in a three step procedure:
washing for 30 minutes in running tap water; immersion in Tween-80 for 2 minutes, followed by three times
washing with distilled water; and finally surface sterilization was performed with 0.1% HgCl2 for 3 minutes,
followed by three times washing with sterile distilled water.