In addition, 0.5
and 1 mg adult−1 (approximately 0.5 and 1 μl, respectively) were directly applied to the adults without dilution in ethyl alcohol. Both treated and control insects (60 adults total per dose) were transferred to
glass vials containing rice and an artificial diet, and were maintained
in a test room at 25 ± 1 °C, 45–50% relative humidity, and a 16:8 h
light:dark photoperiod. Adults were considered dead if appendages
did not move when observed under a microscope (×40)