samples were analyte by UHPLC-MS-MS when a high sensitive analysis was required.a 5 ul inject volume and a mobile phase flow rate of 0.4 ml/min were employed. The mobile phase consisted of 0.1 % formic acid in water and methanol.the gradient started at 10% methanol,was linearly increased to 95% in 4 min,and then maintained for 1 min.acquisition were preformed using 3.5 kV capillaty voltage,120 c° source temperature and 350 c°desolvation temperature.Multiple reaction monitoring mode was adjusted for the MS-MS determination of each analyte.