In our
experiment the different concentration of capsaicin
solution were reduced to give blue colour and the
intensity of the colour was directly proportional to the
concentration of the capsaicin (Fig 1). Qualitative analysis
of the extracts was done by thin layer chromatography.
TLC method is provides a chromatographic plant extract
fingerprint32. In our study the capsaicin spot was
analogous to the reference spot confirming the
qualitative test. The HPLC chromatogram was obtained
with a commercial capsaicin using absorbance at 280 nm.
The sample gave a chromatogram of capsaicin and
dihydrocapsaicin along with minor amounts of the other
capsaicinoids