Scanning electron microscopy
Scanning electron micrographs of the surface of the okara
cookie doughs were recorded by scanning electron microscopy
(JSM-5300, Japan Electric, Tokyo, Japan). The cookie dough samples
were freeze-dried, and stored in a vacuum-sealed wrap until used
within 1 week. The freeze-dried cookie dough was cut into a 10 mm
square, sputter coated with gold (4 min, 2 mbar), transferred to a
microscope, where it was observed at 30 kV and
75 magnification.