J. curcas PZMD16 was crossed to J. integerrima S001
and hybrids F1 lines were generated. Then a BC1F1
population was constructed consisting of 296 individuals
derived from the backcross between PZMD16 used as
recurrent parent line and an F1 line named as CI7041.
The population and parental lines were planted under
standard growth conditions with spacing 2 m X 2 m in
2008 in experimental field of Lim Chu Kang farm, Singapore.