Four varieties of lima beans (Phaseolus lunatus L. WALP) seeds with accession number NSWP100, NSWP98, NSWP47 and NSWP96 were obtained from the Institute of Agricultural Research and Training
(IAR&T), Ibadan, Nigeria. The cleaned lima beans were cracked with hammer mill, soaked in water for 3 h and dehulled. Hull was dried at (60 ± 2 °C) for 24 h, milled and sieved to give 40 mm mesh size flour and kept in airtight plastic containers at 4 °C prior to use. Chemicals used were of analytical grade.