A number of preliminary in vitro and in vivo studies with the water-soluble extract of
M. charantia and its various purified fractions have shown anti-cancer activity against
human bladder carcinomas and breast cancers (Amila et al, 2000; Zhu, 1990). Recent,
subcritical water extract has become an alternative for extraction of herbal plants
(Ayala et al. 2001). The subcritical water extract of M. charantia potentially shows
anti-tumour, anti carcinogenic and anti-inflammatory effects (Lee et al. 2003; Horax et
al. 2005). The dried fruit of M. charantia can be pulverized into a fine powder in a
grinder and stored at 4°C. This powder and the extracts of the powder can be employed
for chemotherapeutic studies (Zhu, 1990; Pitipanponga et al. 2007)