Plant materials: The commercial turmeric roots were washed under running tap water to remove surface pollutants and shadow
dried. The dried sample was powdered were stored in dark sealed glass bottles to protect from humidity and light and used for further
studies.
Solvent used: Different organic solvents chosen with high polar, medium polar, low polar such as water, acetone, methanol and
petroleum ether were used. Suitable solvent for extraction were analysed.
Conventional extraction techniques: Six conventional extraction techniques as given below were used to evaluate the suitable
extraction method of Curcuminoids from Curcuma longa.
Homogenization: 1 gram of turmeric was weighed accurately and was ground well in a mortor & pestle along with different organic
solvents such as water, acetone, methanol and petroleum ether. The solvents were filtered through whatmann filter paper and the
filterate was collected and kept in dark overnight. Solvent was evaporated and the dried extract was calculated and solubilised in
DMSO.
Single extraction: 1 gram of turmeric from Curcuma longa was weighed accurately and extracted using different polar solvents. The
resultants were centrifuged at 10,000 rpm for 5 minutes at room temperature and the supernatants were collected. This procedure was
repeated until the residue become colourless. The supernatant was collected and kept in dark overnight. The dried extract was
weighed, yield was calculated and solubilised in DMSO and stored.
Sequential extraction: About 1 gram of turmeric was extracted with 10 ml of distilled water centrifuged at 10,000rpm for 10 minutes
and the supernatant was collected. After extraction, the residue was reextracted by the addition of 10 ml of methonal, centrifuged at
10,000rpm for 10 minutes and the supernatant was collected after the methonal extraction the residue was reextracted by the addition