This research studied the effect of different pretreatment methods from sugarcane shoots and leaves on enzymatic saccharification and ethanol production. The analysis of the chemical composition of sugarcane shoots and leaves consist of 35.2% cellulose, 23.43% hemicellulose, 12.6% lignin and 6.59% ash on dry solid (DS), three pretreatment methods had been investigated and compared, including: (1) 2% w/v NaOH solution autoclaving pretreatment; (2) 2% w/v H2SO4autoclaving pretreatment and (3) two steps of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO4 solution autoclaving pretreatment, the best result for ethanol production was obtained when 15 g DS of sugarcane shoots and leaves was pretreated by using two step of 2% w/v NaOH solution autoclaving followed by 2% w/v H2SO solution autoclaving. The highest ethanol concentration 30.40 g/L. was obtained from reducing sugar 89.25 g/L at 48 h. Moreover, the washing step of solid residue after pretreatment could reduce furfuraland hydroxymethylfurfural (HMF) in all pretreatment methods when compared to unwashing solid residue after pretreatment, the three pretreatment methods.