Comparing MS and
N6 medium, MS was found better for callus induction and further somatic embryo
development. Callus induction began with the enlargement of the scutellar tissue. The
development of primary callus took three weeks from inoculation of explants. The
nature of calli was quite different from each other inducing from mature and immature
embryos. Calli induced from mature embryos were whitish in colour, soft and spongy.
On the other hand, calli induced from immature embryos were yellowish, more nodular
and comparatively hard (Figure 1A).