Fig. 3. Anti-microbial activity analysis of mutated PFLP protein in vitro. Bacterial suspensions of E. carotovora subsp. carotovora (1 104 cfu/ml) were incubated with 0.2 mM of PFLP, PFLP-GC or PFLP-A549 respectively
in the 1/4NB medium. PFLP pre-treated with boiling water for 15 min (boPFLP), bovine serum albumin (BSA) and sterilized water (water) were used as the controls. The bacterial growth was measured by spectrophotometer O.D.600 at a different time point. Vertical bars represent standard deviations (n = 4).