It can be concluded that pre-treatments of air-drying temperature, particle size, enzyme concentration and extraction time prior to SC-CO2 extraction strongly influence the oil yield and the content of b-carotene and lycopene of the extracts. Results indicated that the highest oil yield was obtained when using enzyme at 0.1% (w/w) concentration for pre-treatment before air-drying. Furthermore, the drying temperature of 50 C and mean particle size of 0.45 mm were the most suitable pre-treatments for the laboratory unit employed. Under these conditions, the high Gac oil yield of about 34%, and the high content of b-carotene (83 mg/100 mL oil) and lycopene (508 mg/100 mL oil) after the extraction time of 120 min were obtained.