The main culture was started by inoculating 5 v/v% of the seed culture. These culture media used in cultivations were sterilized at 121◦C in an autoclave for 30 min and poured into Erlenmeyer flasks (Hestrin & Schramm, 1954). HSculture was incubated statically for 10 days at 28◦C while HWE culture was incubated statically under different temperatures (26◦C,28◦C and 30◦C) and different pH levels (5, 6, 7 and 8).