Determination of effect of phytohormones and amino sugars on CDA activities of C. gloeosporioides
SmCs were carried out in flasks with 50 mL of medium for screening inductors. The medium was inoculated with 1 × 107 spores g of nutrient−1 and incubated at 28 °C. Samples were taken in duplicates every 24 h up to 96 h. The cultures were filtered and biomasses were determined as dry weight. The filtrates from cultures were used as crude enzyme solutions. Media were supplemented with several compounds including phytohormones, GA, ABA and IAA, at 0.23, 2.3, 23 and 230 mg L−1 and Glc at same range of concentrations also 770 and 2000 mg L−1, samples were taken at 72 h. The time course of CDA of SmC in media with added selected concentrations of compounds experiment was carried out over 96 h.