The second generation technology of bioethanol production consist of two main processes, which are hydrolysis of cellulosic and hemicellulosic to deliver reducing sugar and sugar fermentation into ethanol [5]. After pretreatment, which removing lignin and enhancing cellulose fraction on biomass, saccharification and fermentation are able to conduct at one flask that called simultaneous saccharification and fermentation (SSF) [6]. The advantage of this method is converting monosaccharide into ethanol simultaneously, reducing equipment cost and reducing contamination [7].