broken rice from 10 g per randomly collected sample of milled
brown rice were recorded.
Trichoderma colonization of rice roots
The percentages of rice roots colonized by T. asperellum were
detected by collecting rice root systems from harvested plants of
each replication. Roots were washed three times under running
tap water, cut to a single piece, blotted dry using sterilized tissue
paper and placed on Martin's medium supplemented with
100 parts per million rifampicin. After incubation under light at
room temperature (25e30 C) for 3e5 d, the numbers of rice roots
colonized with T. asperellum were recorded