Then, the mixture was exposed to vortex mixing for 40 min followed by centrifugation at 3,500 rpm for 10 min to achieve the separation of water-soluble and water-insoluble fractions.
Then, the mixture was exposed to vortex mixing for 40 minfollowed by centrifugation at 3,500 rpm for 10 min to achieve theseparation of water-soluble and water-insoluble fractions.