Proteins in the FE were separated by isoelectric focusing (IEF) using the Multiphor II electrophoresis system (Pharmacia-LKB
Biotechnology) according to the manufacturer's instructions. Focusing was carried out on a 6% polyacrylamide gel with
ampholytes in the 3e10 pH range. Endocellulase isoforms were detected in acrylamide (AA) (7.5%) gel with copolymerized 1% CMC, previously printed from IEF gel for 55 min. The cellulase zymogram