In summary, this is the first report of establishing an
efficient and rapid plant regeneration system from anther
explants of T. patula. In this work, the effects of genotype,
temperature pretreatment, plant growth regulators, light
and sucrose concentration were studied and the result
showed that when T. patula 21605 anthers with
microspores at the mid to late uninucleate stage were
cultured on MS basal medium containing BA (2.2 µM)
and NAA (1.82 µM or 2.7 µM) and 3% sucrose
concentration under light, optimum regeneration could be
obtained, and frequencies of callus induction and shoot
regeneration were 100 and 70.5%, respectively. In
addition, the haploid plants were not obtained, may be
the callus were obtained from the anthers wall. The
results also demonstrated that frequency of plant
regeneration from anther culture were highest when
microspores were at the mid to late uninucleate stage.
This system was advantageous in that the calli could be
maintained or induced to differentiate by light control, and
plants could be regenerated as needed. This regeneration
system is therefore useful for developing an
efficient genetic transformation system, and an efficient
doubled haploid plant induction system could be
established after further modification of the culture
parameters.
In summary, this is the first report of establishing anefficient and rapid plant regeneration system from antherexplants of T. patula. In this work, the effects of genotype,temperature pretreatment, plant growth regulators, lightand sucrose concentration were studied and the resultshowed that when T. patula 21605 anthers withmicrospores at the mid to late uninucleate stage werecultured on MS basal medium containing BA (2.2 µM)and NAA (1.82 µM or 2.7 µM) and 3% sucroseconcentration under light, optimum regeneration could beobtained, and frequencies of callus induction and shootregeneration were 100 and 70.5%, respectively. Inaddition, the haploid plants were not obtained, may bethe callus were obtained from the anthers wall. Theresults also demonstrated that frequency of plantregeneration from anther culture were highest whenmicrospores were at the mid to late uninucleate stage.This system was advantageous in that the calli could bemaintained or induced to differentiate by light control, andplants could be regenerated as needed. This regenerationsystem is therefore useful for developing anefficient genetic transformation system, and an efficientdoubled haploid plant induction system could beestablished after further modification of the cultureparameters.
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