In the fourth week it finally extended to become a young sporophyte (Figure 1H). Intact separable sporophytes were collected after culturing for eight weeks. We recorded an average production of 25+/- 2.18 sporophytes from one single GGB (initial size 8-10 mm) through this procedure.
However, medium supplemented with activated charcoal (which we expected to facilitate the removal of carried-over PGR) significantly inhibited sporophyte development.