In a different approach, Nantaphol et al. developed a cholesterol biosensor by coupling a paperanalytical device with a commercial boron-doped diamond electrode [41]. The paper section hadcarbon counter and Ag/AgCl reference electrodes screen-printed on its hydrophilic areas. The backof the paper was applied onto double-sided adhesive tape (with a hole cut through the overlayingelectrode area) which was then attached to the working electrode–a boron-doped diamond sheet.This electrode was modified with electrodeposited AgNPs, in order to increase the electrochemicalspecific surface area and to improve the conductivity and the electrocatalytic activity. To completebiosensor construction, ChOx was drop casted and dried atop of the hydrophilic zone of the papersupport. The H2O2generated by the enzymatic reaction in the presence of cholesterol was reduced atthe NPs modified working electrode, generating a current signal that was measured by amperometry.The biosensor exhibited a high selectivity for cholesterol detection and an excellent performance interms of detection limits (comparable to other previously reported biosensors) and for real sampleanalysis (bovine serum) [41]