The preliminary result of the antimicrobial activity in 5 herbal plants extract namely Gotu Kola , Finger grass , Commelinaceae ,Turmeric and Mangosteen. The antibacterial Staphylococcus aureus In food borne diseases. The herb is used to extract water and ethanol 95%. by Agar well diffusion method. The inhibition is different The antibacterial effect on performance Staphylococcus aureus. Herbal extract with ethanol 95% effective in inhibiting infection is one example Mangosteen extract. The extracts were distilled water can crumpled on one sample is Finger grass. The Mangosteen extract with 95% ethanol The mean diameter of the power to withhold an average of 14 mm and Finger grass extracts 8.3 mm. When the extracts were compared with the average diameter Amoxicillin drug over the 5 kinds of herbal extract.