The 4a-glucanotransferase
gene, cloned by Park et al. (2007),
encoded the TA
aGT enzyme. The plasmid was expressed in E. coli
MC1061, and the target enzyme was isolated using a nickelnitrilotriacetic
acid (Ni-NTA) column (Qiagen, Hilden, Germany)
(Park et al., 2007). The purity of TA
aGT enzymes was confirmed by
SDS-PAGE.