Cows were housed in individual pens and individually
fed concentrate at 1.5% BW. All cows were fed ad libitum
of ruzi grass with water and a mineral-salt block. Feed
intake of concentrate and roughage were measured
separately and refusals recorded. The experiment was
run in four periods, each experimental period lasted for
21 days, the first 14 days for treatment adaptation and for
feed intake measurements whist the last 7 days were for
sample collections of rumen fluid and faeces. Body
weights were measured daily during the sampling
period prior to feeding.