The same relationship between Tleaf and gas-exchange measurements were found using the values of Tleaf–air(Fig. 4D–F). Although these relations were not similar for each timing measurement, there was a grouping of dataset , mainly for the data from the beginning of the experiment (0 DAT) in relation to the rest. When these same relationships were calculated using the average values, as described in the model of Eq. (1), the optimum ranges of Tleaf–air related to gas exchange measurements were identified(Fig 5, Table 4), that is, those in which a minimal increase was detected in gas-exchange responses. According to these results,Tleaf–air values below to −2◦C would be accompanied with maxi-mum values of An and gs the maximum values of E would be reached for values of Tleaf–air close to −4◦C.