Samples (20 gm) in duplicate from the irradiated as
well as the non-irradiated batch were aseptically homogenised
for 2min in a sterile stomacher bag containing
180 ml of sterile saline using Stomacher 400 Lab
Blender (Seward Medical, UK). Serial dilutions of the
homogenate were prepared and colony-forming units
were determined. Media employed for determining
counts of S. aureus, C. sporogenes and B. cereus were
Baird-Parker agar, sodium sulphite polymixin sulphadiazine
(SPS) agar and B. cereus agar (HiMedia, India)
respectively. C. sporogenes plates were incubated in
anaerobic conditions in an Anaerobic Ja