i) Dispense 0.025 ml of PBS into each well of a plastic V-bottomed microtitre plate.
ii) Place 0.025 ml of virus suspension (i.e. infective allantoic fluid) in the first well. For
accurate determination of the HA content, this should be done from a close range of an
initial series of dilutions, i.e. 1/3, 1/4, 1/5, 1/6, etc.
iii) Make twofold dilutions of 0.025 ml volumes of the virus suspension across the plate.
iv) Dispense a further 0.025 ml of PBS to each well.
v) Dispense 0.025 ml of 1% (v/v) chicken RBCs to each well.
i) Dispense 0.025 ml of PBS into each well of a plastic V-bottomed microtitre plate.ii) Place 0.025 ml of virus suspension (i.e. infective allantoic fluid) in the first well. Foraccurate determination of the HA content, this should be done from a close range of aninitial series of dilutions, i.e. 1/3, 1/4, 1/5, 1/6, etc.iii) Make twofold dilutions of 0.025 ml volumes of the virus suspension across the plate.iv) Dispense a further 0.025 ml of PBS to each well.v) Dispense 0.025 ml of 1% (v/v) chicken RBCs to each well.
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