The mixed substrates used for SSF consisted of: (1) for kefir,
30 ml orange pulp, 10 ml molasses, 10 ml potato pulp, 100 ml
whey, 50 ml distilled water, 60 g BSG and 25 g MSR, (2) for S. cerevisiae
AXAZ-1, 100 ml orange pulp, 50 ml molasses, 50 ml distilled
water and 70 g BSG, and (3) for K. marxianus IMB3, 100 ml orange
pulp, 10 ml molasses 10 ml potato pulp, 30 ml whey, 50 ml distilled
water and 80 g BSG. The amount of BSG and MSR was chosen
so that the mixed substrates to have 70–80% w/w moisture content.
The pH was adjusted to 5.5 by a 2 N NaOH solution for S. cerevisiae
and kefir and to 7.0 for K. marxianus. The substrates were
sterilized by autoclaving for 15 min at 120 C and 1.5 Atm. After
cooling, they were spread into petri dishes, inoculated with 1 g of
harvested culture and incubated for 4 days at 30 C without any
agitation.
The mixed substrates used for SSF consisted of: (1) for kefir,30 ml orange pulp, 10 ml molasses, 10 ml potato pulp, 100 mlwhey, 50 ml distilled water, 60 g BSG and 25 g MSR, (2) for S. cerevisiaeAXAZ-1, 100 ml orange pulp, 50 ml molasses, 50 ml distilledwater and 70 g BSG, and (3) for K. marxianus IMB3, 100 ml orangepulp, 10 ml molasses 10 ml potato pulp, 30 ml whey, 50 ml distilledwater and 80 g BSG. The amount of BSG and MSR was chosenso that the mixed substrates to have 70–80% w/w moisture content.The pH was adjusted to 5.5 by a 2 N NaOH solution for S. cerevisiaeand kefir and to 7.0 for K. marxianus. The substrates weresterilized by autoclaving for 15 min at 120 C and 1.5 Atm. Aftercooling, they were spread into petri dishes, inoculated with 1 g ofharvested culture and incubated for 4 days at 30 C without anyagitation.
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