Membrane stability index
Membrane stability index was estimated 100-mg leaf material
using two sets of test tubes containing 10 ml of
double distilled water (Sairam 1994). One set was heated
at 40 °C for 30 min in a water bath, and the electrical
conductivity of the solution was recorded using conductivity
meter (C1). Second set was boiled at 100 °C on a
boiling water bath for 10 min, and its conductivity (C2)
was measured as above. Membrane stability index (MSI)
was calculated as: