Preparation of Bacteria
Bacteria were grown on trypticase soy agar plates overnight at 37°C.
Immediately before infection, bacteria were suspended in minimal
essential Eagle medium (50% [vol/vol]) in Hams F-12 (BioWhittaker,
Walkersville, MD) tissue culture medium (EMEM/Hams F-12) to a final
approximate concentration of 5 106 CFU/mL. P. aeruginosa clinical
isolates 6294 and 6206 were used as positive controls for invasion and
cytotoxicity assays, respectively.