Measurement of lipid peroxidation was done by the method based on the reaction of 2-thiobarbituric acid (TBA) with reactive species derived from lipid peroxidation, particularly malondialdehyde (MDA). Detection of 2-thiobarbituric acid reactive substances (TBARS) was carried out by a colorimetric assay described. with some modifications (Espindola et al., 2003).