The effect of auxins on the induction collusing from shoot tips and leaves were different. The callus developed was white in
Table 2: Effect of various concentration of cytokinins and combination of Cytokinin and auxin on callus induction and shoot differentiation from shoot tip Culture of Annona reticulata
colour in nature. Only callusing was evident but no symptoms of organogenesis, was seen in medium containing 2, 4-D (Fig. 1) 2, 4 –D at 2.25 μm gave maximum shoot initiation shoot number and mode number, identical results were obtained by Sen and Sharma (1991) in Withania – Somnifera callus induction and organogensis in Sesbania spp. were obtained by Khattar and Mohan Ram (1982, 1983) and Subhan et al., (1998) using B5. The best result was encountered at 2.5mg/L concentration of auxins in the present work. Hundred percent cultures exhibited callusing at this concentration. IAA was proved to be more effective than IBA in inducing callus from shoot tip culture. In the shoot tip culture on MS medium containing IAA (0.5 mg/L) the callusing from its anterior end was found. Incorporation of NAA on MS medium at concentration raging from 0.5 to 10 mg/L yielded callusing from cut end of shoot tips. 5 mg /L was recorded as the best concentration for callus induction and 100% culture exhibited callusing (Fig. 2; Table 1).