P. clarkii specimens were captured in a heavily polluted industrial and technological area in Central Italy (IND) as well as in the nearby (about 35 km) Lake Trasimeno (TRA). The IND sampling site was schematically described in Fig. 1. Four sampling stations (St. 1–4) were set on two streams (A and B in Fig. 1), which define the IND basin. The hydrological
regime of the streams is strongly dependent on precipitation. The four stations were located approximately at 1.5 km from the centreof the industrial area (Fig. 1).
ake Trasimeno (TRA) is an important freshwater ecosystem in Italy:the largest shallow lake and the fourth largest lake in surface. For its ecological value this biotope is designated as “Natura 2000” Site (Directive No 43 (EEC, 1992): IT5210018 and IT5210070) and as Regional Park. The sediment contamination status of the TRA site was previously described
from Di Veroli et al. (2012). Recently the red swamp crayfish population of the Lake Trasimeno has rapidly increased and has become object of professional fishery (Dörr et al., 2006).