2.1. Preparation of SF and ADA
In this experiment, The SF solutions were prepared by the method mentioned in the literature [16] and then lyophilized for
about 48 h. Briefly, bombyx morisilk were treated twice with 0.5%(w/w) NaHCO3 solution at 70 ◦C for 1 h and then rinsed with 70 ◦C distilled water to remove sericin. Degummed silk was dissolved in a mix solvent system of CaCl2/CH3CH2OH/H2O (mole ratio, 1:2:8) at 70 ◦C for 6 h and filtered to get a SF solution. The SF solution was dialysis and then lyophilized. ADA was prepared with slight modification for better oxidation as the method in our previously study.
[17].