The animal care and experimental procedures used in this studycomplied with EU Directive 2010/63 and Spain's Royal Decree 53/2013, concerning the protection of animals used for scientific research.All procedures involving animals were developed by staff at Frigolouro,a company complying with the requirements of welfare and protectionof animals set in Spain's Royal Decree 54/1995, which was further repealed by Spain's Royal decree 37/2014.