A standard curve was generated using duplicates of ten-fold
serial dilutions of the A. carbonarius 5010 DNA. The logarithmic
values for the starting quantity of template (5 to 5 104 ng) were
plotted against the corresponding Ct values, which were estimated
using the ABI PRISM 7900 software. The amplification efficiency
was calculated from the slope of the standard curve, using:
E ¼ 101/slope and % Efficiency ¼ (E1) 100.