2.8. Statistical analysis
All the results were subjected to the analysis of variance
(ANOVA) using a Statgraphics Plus 5.0. (STATPOINT TECHNOLOGIES
INC., USA) software. Differences were considered as significant
at p < 0.05. Moreover, before the ANOVA procedure the normality
of the distribution was evaluated. Homogeneous group were
determined using Duncan’s multiple range tests with probability
level set at 0.05