Bacterial spores have demonstrated pressure resistance and
the mechanisms through which they are inactivated are different
from these for the vegetative cells. It has been suggested
that the spore proteins are protected against solvation and
ionization (21, 45). Microbial spores could be inactivated by
chosen suitable conditions for high pressure treatment: higher
pressure and long exposure time at elevated temperature (21).
It was assumed that pressure caused inactivation of spores by
first initiating germination and then inactivating germinated
forms. The spore germination could be induced by hydrostatic
pressure of 100-300 MPa and resultant vegetative cells are
sensitive to environmental conditions (33, 34).