The aim of this study was to assess the effect of different preservatives on the physico-chemical quality of Soy milk stored at different temperatures. The utilization of soy bean for the production of soy milk was evaluated. Soy milk was extracted from whole and dehulled seed, pasteurized and fermented. Physicochemical qualities of the soy milk samples were evaluated to determine the physical and chemical quality of the products. All the samples were treated with potassium sorbate and sodium benzoate (in combination) with propyl gallate and Ascorbyl palmitate (singly and in combination), organoleptically evaluated and stored at ambient temperature and 4 ± 2OC for 16 days and were subjected to physical examination and chemical analyses such as pH and titratable acidity (TTA). There was a gradual decrease in the pH and an increase in the total titratable acidity of the samples. For both pH and TTA, there was significant difference (P